Thermo Fisher Scientific Phospho-Tie2 (Tyr992) Polyclonal Antibody
Phospho-Tie2 (Tyr992) 폴리클로날 항체로 인간, 마우스, 랫트에 반응. WB, IHC, ICC, ELISA 등 다양한 응용 가능. Protein A로 정제된 액상 항체로 1 mg/mL 농도. -20°C 보관, 연구용 전용 제품.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:400 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic phosphopeptide derived from human Tie2 around the phosphorylation site of Tyr992 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The TEK receptor tyrosine kinase is expressed almost exclusively in endothelial cells. TEK signaling is critical for endothelial cell–smooth muscle cell communication in venous morphogenesis, and defects in TEK are associated with inherited venous malformations.
The ligand angiopoietin-1 binds to this receptor and mediates a signaling pathway important for embryonic vascular development.
Immunoblotting showed that TIE2 expression was increased by thyroid-stimulating hormone and agents that elevate intracellular cAMP.
Hematopoietic stem cells (HSCs) expressing TIE2 are quiescent and antiapoptotic, forming a population that adheres to osteoblasts in the bone marrow niche.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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