Thermo Fisher Scientific Ribophorin I Polyclonal Antibody
Ribophorin I 단백질을 인식하는 Rabbit Polyclonal 항체로, WB, IHC(P), ELISA에 사용 가능. Human, Mouse, Rat 반응성. Affinity 정제 및 PBS/glycerol buffer에 보관. -20°C에서 보관하며, ER 막 단백질 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing amino acids 20–280 of human RPN1 (NP_002941.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.19 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2805018 |
Product Specific Information
Immunogen sequence:
APGSASSEAP PLINEDVKRT VDLSSHLAKV TAEVVLAHLG GGSTSRATSF LLALEPELEA RLAHLGVQVK GEDEEENNLE VRETKIKGKS GRFFTVKLPV ALDPGAKISV IVETVYTHVL HPYPTQITQS EKQFVVFEGN HYFYSPYPTK TQTMRVKLAS RNVESYTKLG NPTRSEDLLD YGPFRDVPAY SQDTFKVHYE NNSPFLTITS MTRVIEVSHW GNIAVEENVD LKHTGAVLKG PFSRYDYQRQ PDSGISSIRS F
Positive Samples: HL-60, HepG2, SKOV3, MCF7, BxPC-3, Mouse liver, Rat liver
Cellular Location: Endoplasmic reticulum membrane, Melanosome, Single-pass type I membrane protein
Target Information
Ribophorins 1 and 2 are abundant and highly conserved glycoproteins located in the endoplasmic reticulum, participating in ribosome binding. Mammalian oligosaccharyltransferase activity is associated with a protein complex composed of RPN1, RPN2, and an oligosaccharyltransferase protein. RPN1 is a component of the proteasome base, and its ubiquitin-like (UBL) domain interacts with proteasomes through the leucine-rich repeat domain. The RPN1 gene maps to chromosome 3, while RPN2 maps to chromosome 20.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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