Thermo Fisher Scientific Phospho-AKT Pan (Thr308, Thr305, Thr309) Recombinant Rabbit Monoclonal Antibody (5E9G5)
Recombinant rabbit monoclonal antibody recognizing phosphorylated AKT (Thr308, Thr305, Thr309). Validated for WB and ELISA. High specificity and sensitivity with positive controls NIH/3T3 and C6. Ideal for studying AKT signaling and cancer research.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| ELISA | 1 µg/mL |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 5E9G5 |
| Immunogen | A synthetic phosphorylated peptide around T308 of human Akt (NP_0051542) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol, 0.05% BSA |
| Contains | 0.05% ProClin 300 |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2912101 |
Product Specific Information
Positive test controls include NIH/3T3 and C6 cells.
Immunogen sequence: MKTFC
Target Information
AKT, also known as protein kinase B (PKB) or RAS-alpha, is a serine/threonine kinase involved in diverse biological processes including metabolism regulation, cell survival, and growth. It is activated by insulin, PI3K, IGF1, and other growth factors. AKT promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of targets such as forkhead transcription factors and caspase-9.
The AKT pathway is a major focus in cancer drug discovery.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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