ELK Biotechnology ZP4 rabbit pAb
ZP4 단백질을 인식하는 토끼 다클론 항체로, Western blot과 ELISA에 적합합니다. 인공 합성 펩타이드 항원으로 제작되었으며, 인간, 생쥐, 랫트 시료에서 반응합니다. 65kDa 밴드 검출, -20°C에서 1년 보관 가능합니다.
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ELK Biotechnology ZP4 rabbit pAb
제품 정보
| 항목 | 내용 |
|---|---|
| Product name | ZP4 rabbit pAb |
| Alternative Names | ZP4; ZPB; Zona pellucida sperm-binding protein 4; Zona pellucida glycoprotein 4; Zp-4; Zona pellucida protein B |
| Applications | WB; ELISA |
| Recommended Dilutions | Western Blot: 1/500 - 1/2000 ELISA: 1/10000 Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ZP4. AA range: 231-280 |
| Host | Rabbit |
| Storage | -20°C / 1 year |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1 mg/ml |
| Observed Band | 65 kD |
| GeneID (Human) | 57829 |
| Human Swiss-Prot No | Q12836 |
| Cellular Localization | [Processed zona pellucida sperm-binding protein 4]: Zona pellucida; Cell membrane; Single-pass type I membrane protein |
| Species Reactivity | Human; Rat; Mouse |
Background
The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. The nascent protein contains an N-terminal signal peptide sequence, a conserved ZP domain, a consensus furin cleavage site, and a C-terminal transmembrane domain. It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix. However, the requirement for furin cleavage in this process remains controversial based on mouse studies. Previously, this gene has been referred to as ZP1 or ZPB and thought to have similar functions as mouse Zp1. However, a human gene with higher similarity and chromosomal synteny to mouse Zp1 has been assigned the symbol ZP1 and this gene has been.
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