Thermo Fisher Scientific Connexin-43 Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

Reconstitution: 25 µL, 50 µL, or 0.2 mL double distilled water (DDW) depending on the sample size.
The antibody ships as a lyophilized powder at room temperature. Upon arrival, store at -20°C.
The reconstituted solution can be stored at 4°C for up to 1 week. For longer storage, prepare small aliquots and store at -20°C.
Avoid multiple freeze-thaw cycles. Centrifuge all antibody preparations before use (10,000 × g, 5 min).

Target Information

Connexin 43 (Cx43) is a member of the gap junction protein family. Connexins assemble as hexamers and are transported to the plasma membrane to form hemichannels, which pair with adjacent cell hemichannels to create gap junctions. These structures facilitate intercellular communication, crucial for cell growth regulation and development.

Phosphorylation of Cx43, particularly at Ser368 by protein kinase C (PKC), modulates gap junction assembly and function, reducing cell-to-cell communication. Src kinase can also phosphorylate Cx43, altering its communication properties.

Connexin 43 is the predominant gap junction protein in the heart, essential for synchronized cardiac contraction and embryonic development. It is regulated by multiple kinases influencing myocardial coupling. A related pseudogene, GJA1P, is located on chromosome 5. Mutations in the GFAP gene are associated with Charcot-Marie-Tooth disease, oculodentodigital dysplasia, and heart malformations. Alternatively spliced GFAP variants have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.