Thermo Fisher Scientific LEF1 Monoclonal Antibody (5A3)
LEF1 단백질을 인식하는 Mouse monoclonal antibody로 Western blot, ICC/IF, ELISA에 사용 가능. Human 시료에 반응하며, Affinity chromatography로 정제된 액상 형태. Wnt 신호전달 및 세포 분화 연구에 적합.
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- H00051176-M03
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–5 µg/mL |
| Immunocytochemistry (ICC/IF) | 10 µg/mL |
| ELISA | 0.3 ng/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5A3 |
| Immunogen | LEF1 (NP_057353, 14 a.a. ~ 123 a.a.) partial recombinant protein with GST tag (GST tag MW: 26 kDa) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | See label |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Protein Sequence:
DPELCATDEM IPFKDEGDPQ KEKIFAEISH PEEEGDLADI KSSLVNESEI IPASNGHEVA RQAQTSQEPY HDKAREHPDD GKHPDGGLYN KGPSYSSYSG YIMMPNMNND
Target Information
This gene encodes a transcription factor belonging to a family of proteins that share homology with high mobility group protein-1. The encoded protein binds to a functionally important site in the T-cell receptor-alpha enhancer, conferring maximal enhancer activity. It is involved in the Wnt signaling pathway and may function in hair cell differentiation and follicle morphogenesis. Mutations in this gene are associated with sebaceous tumors and other cancers, including androgen-independent prostate cancer. Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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