Thermo Fisher Scientific CD117 (c-Kit) Monoclonal Antibody (2B8), PerCP-eFluor 710, eBioscience

Thermo Fisher Scientific CD117 (c-Kit) Monoclonal Antibody (2B8), PerCP-eFluor 710, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.125 µg/test
Publications: View 62 publications

Product Specifications

Additional Formats Available

• Unconjugated
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395 / 563 / 615 / 661 / 737
• Brilliant Violet 421 / 480 / 605 / 650 / 786
• eFluor 450
• FITC
• PE / PE-Cyanine5 / PE-Cyanine7 / PE-eFluor 610
• Super Bright 436 / 600 / 645 / 702 / 780
• Request custom conjugation

Product Specific Information

The 2B8 monoclonal antibody reacts with mouse CD117 (c-Kit receptor), also known as Steel factor receptor or stem cell factor receptor. This 145-kDa tyrosine kinase receptor is expressed in hematopoietic progenitor cells, mast cells, and plays a role in cell proliferation and differentiation.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Flow cytometry on C57Bl/6 bone marrow cells (≤0.125 µg/test)
• Recommended: Titrate for optimal performance
• Excitation/Emission: 488 nm / 710 nm (Blue Laser)
• Recommended Filter: 710/50 bandpass (or 695/40 alternative)
• Fixation: Compatible with IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-54)
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

KIT (c-KIT) is a proto-oncogene encoding a type 3 transmembrane receptor for mast cell growth factor (stem cell factor, SCF). It regulates growth and activation of hematopoietic and non-hematopoietic cells. Mutations in KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism.

Upon SCF binding, c-Kit dimerizes and undergoes autophosphorylation, activating downstream pathways such as Ras/ERK, PI3K, PLC-γ, and JAK/STAT. KIT receptor activation is essential for hematopoiesis, stem cell maintenance, and gametogenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지