Thermo Fisher Scientific CD117 (c-Kit) Monoclonal Antibody (2B8), PerCP-eFluor 710, eBioscience
Flow cytometry용 CD117 (c-Kit) 단일클론 항체로, PerCP-eFluor 710 형광체 결합. 마우스 및 돼지 반응성, Rat IgG2b 아이소타입. 4°C 암소 보관, 혈액세포 및 조혈줄기세포 분석에 적합.
- 카탈로그번호
- 46-1171-8x (2개 옵션)
- 판매단위
- pk
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2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD117 (c-Kit) Monoclonal Antibody (2B8), PerCP-eFluor 710, eBioscience
Thermo Fisher Scientific CD117 (c-Kit) Monoclonal Antibody (2B8), PerCP-eFluor 710, eBioscience
Applications
- Flow Cytometry (Flow)
Tested Dilution: 0.125 µg/test
Publications: View 62 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse, Pig |
| Published Species | Fish, Mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), PerCP-eFluor™ 710, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2B8 |
| Conjugate | PerCP-eFluor™ 710 |
| Excitation / Emission Max | 482 / 708 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1834422 |
Additional Formats Available
- Unconjugated
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395 / 563 / 615 / 661 / 737
- Brilliant Violet 421 / 480 / 605 / 650 / 786
- eFluor 450
- FITC
- PE / PE-Cyanine5 / PE-Cyanine7 / PE-eFluor 610
- Super Bright 436 / 600 / 645 / 702 / 780
- Request custom conjugation
Product Specific Information
The 2B8 monoclonal antibody reacts with mouse CD117 (c-Kit receptor), also known as Steel factor receptor or stem cell factor receptor. This 145-kDa tyrosine kinase receptor is expressed in hematopoietic progenitor cells, mast cells, and plays a role in cell proliferation and differentiation.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Flow cytometry on C57Bl/6 bone marrow cells (≤0.125 µg/test)
- Recommended: Titrate for optimal performance
- Excitation/Emission: 488 nm / 710 nm (Blue Laser)
- Recommended Filter: 710/50 bandpass (or 695/40 alternative)
- Fixation: Compatible with IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-54)
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
KIT (c-KIT) is a proto-oncogene encoding a type 3 transmembrane receptor for mast cell growth factor (stem cell factor, SCF). It regulates growth and activation of hematopoietic and non-hematopoietic cells. Mutations in KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism.
Upon SCF binding, c-Kit dimerizes and undergoes autophosphorylation, activating downstream pathways such as Ras/ERK, PI3K, PLC-γ, and JAK/STAT. KIT receptor activation is essential for hematopoiesis, stem cell maintenance, and gametogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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