Applied Biosystems™

Arcturus™ Paradise™ PLUS QC Kit

Formalin-fixed paraffin-embedded (FFPE) samples introduce unique challenges for gene expression profiling and gene expression quantitation, including chemical modification and fragmentation자세히 알아보기

Formalin-fixed paraffin-embedded (FFPE) samples introduce unique challenges for gene expression profiling and gene expression quantitation, including chemical modification and fragmentation of RNA molecules. Archival FFPE samples present additional challenges due to increased RNA degradation over time. As a result, not all FFPE samples contain high quality RNA.

The ARCTURUS™ Paradise™ PLUS Sample Quality Assessment Kit offers a simple solution for evaluating the quality of RNA from Formalin-Fixed Paraffin-Embedded (FFPE) tissues before proceeding with laser microdissection and downstream molecular analysis. The reagents provided in the kit are optimized to enable efficient extraction, isolation, and reverse transcription of total RNA from FFPE tissue scrapes. The RNA quantity and quality is then measured using quantitative real-time PCR (Figure 1).

The RNA quantity and quality are assessed by the presence of intact RNA for a specific message. Two sets of β-actin gene-specific primers designed to amplify short segments of DNA within the β-actin target sequence are used to estimate the amount of RNA in a given sample in relation to β-actin content using the real-time PCR technique. The RNA quantity derived from the 3primer set is used to quantitate the RNA in the FFPE tissue scrape. The ratio of the RNA yield obtained from both sets of PCR primers is the 3⁄5` ratio and is used as an indication of RNA quality.

Key product features:
• Secure Downstream Success with the Paradise™ QC Kit
• Benefit from the Only Complete System for Formalin-fixed Samples

Benefit from the Only Complete System for Formalin-fixed Samples
The Paradise™ PLUS Reagent System is the only available solution that provides all the components necessary to prepare RNA from FFPE tissue samples for gene expression analysis. To ensure successful microarray results, the system includes reagents optimized for exceptional recovery of RNA and superior RNA amplification.

Integrated Systems for Microgenomics
The Paradise™ PLUS Reagent System is validated as part of the ARCTURUS™ Complete System for Microgenomics™, an integrated solution for utilizing small quantities of RNA for gene expression analysis, and features the ARCTURUSXT™ Laser Capture Microdissection (LCM) Instrument to procure pure cell populations. Arcturus` Systems for Microgenomics enable accurate and sensitive microarray assays that reveal molecular otherwise obscured in whole tissue samples or cell mixtures.

For Research Use Only. Not for use in diagnostics procedures.

사양

유형PLUS QC Kit

용도(애플리케이션)Real-Time Quantitative PCR (qPCR)

용도(장비)ArcturusXT™ LCM Instrument

포함cDNA Synthesis Kit, RNA Isolation Kit (RNA Extraction and Purification Reagents, Purification Columns)

제품라인ARCTURUS™, Paradise™

수량48 Samples

샘플 종류Tissue (FFPE)

워크플로우 단계Reverse Transcription, RNA Extraction, Sample QC, RNA Amplification

Unit Size48 samples