Thermo Fisher Scientific Histone H2A.X Polyclonal Antibody
Rabbit polyclonal antibody against human Histone H2A.X for WB, IHC, ICC/IF. Detects DNA damage marker γ-H2AX. Suitable for multiple cell lines. Supplied as liquid, 0.52 mg/mL, stored at -20°C.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence (ICC/IF) | 1:50–1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids 50–143 of human Histone H2AX (NP_0020961) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.52 mg/mL |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2913681 |
Product Specific Information
- Positive test controls: 293T, NIH/3T3, C2C12, Jurkat, Raji, Rat thymus
- Subcellular localization: Chromosome, Nucleus
Immunogen sequence:
VYLAAVLEYL TAEILELAGN AARDNKKTRI IPRHLQLAIR NDEELNKLLG GVTIAQGGVL PNIQAVLLPK KTSATVGPKA PSGGKKATQA SQEY
Target Information
Histone H2A.X (H2AX) is a member of the histone H2A family, one of the four core histones forming the nucleosome core particle. Upon DNA double-strand breaks (DSBs), serine 139 of H2AX is phosphorylated to form γ-H2AX, a marker of DNA damage. This phosphorylation can be detected by Western blotting or immunofluorescence to assess DSB frequency.
Phosphorylation is mediated by phosphatidylinositol 3-kinases, including ATM, DNA-PK, and ATR. H2A.X consists of 143 amino acids and is synthesized in both G1 and S phases. Its mRNA exists in two forms: a 1600-base polyA+ form and a 575-base replication-type form without polyA. The gene maps to human chromosome 11q23.2–q23.3.
Histone H2A.X contributes to nucleosome formation and chromatin structure, and interacts with MDC1 at DNA damage sites.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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