Thermo Fisher Scientific MiTF Recombinant Rabbit Monoclonal Antibody (6S9G4)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000-1:20,000

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

6S9G4

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 402-526 of human MITF (NP_001341533.1). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:MiTF,uniProtId:O75030-1,ncbiNodeId:9606,antigenRange:402-526,antigenLength:526,antigenImageFileName:MA5-51329_MiTF_O75030-1_Rabbit.svg,antigenImageFileNamePDP:MA5-51329_MiTF_O75030-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.227 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 0.05% BSA, 50% glycerol

Contains

0.05% ProClin 300

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_3093734

Product Specific Information

Immunogen sequence: HGLSLIPSTG LCSPDLVNRI IKQEPVLENC SQDLLQHHAD LTCTTTLDLT DGTITFNNNL GTGTEANQAY SVPTKMGSKL EDILMDDTLS PVGVTDPLLS SVSPGASKTS SRRSSMSMEE TEHTC

Target Information

Mi is a basic helix-loop-helix-leucine zipper (b-HLH-ZIP) transcription factor implicated in pigmentation, mast cells and bone development. The mutation of Mi causes Waardenburg Syndrome type II in humans. In mice, a profound loss of pigmented cells in the skin eye and inner ear results, as well as osteopetrosis and defects in natural killer and mast cells. There are two known isoforms of Mi differing by 66 amino acids at the NH2 terminus. Shorter forms are expressed in melanocytes and run as two bands at 52 kDa and 56 kDa, while the longer Mi form runs as a cluster of bands at 60-70 kDa in osteoclasts and in B16 melonoma cells (but not other melanoma cell lines), as well as mast cells and heart.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.