Thermo Fisher Scientific YAP1 Recombinant Rabbit Monoclonal Antibody (5J9N6)
YAP1 단백질을 인식하는 Thermo Fisher Scientific의 재조합 토끼 단일클론 항체로, WB, IHC, ICC, ELISA, IP, ChIP 등 다양한 연구용 응용에 적합합니다. 높은 특이성과 재현성을 제공하며, HEK293 발현 시스템 기반으로 제조되었습니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:20,000–1:80,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:800 |
| Immunocytochemistry (ICC/IF) | 1:200–1:800 |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | 0.5–4 µg antibody for 200–400 µg extracts |
| ChIP assay (ChIP) | 5 µg antibody for 10–15 µg of Chromatin |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 5J9N6 |
| Immunogen | Recombinant fusion protein containing amino acids 155–504 of human YAP1 (NP_001123617.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 0.05% BSA, 50% glycerol |
| Contains | 0.05% ProClin 300 |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_3094113 |
Product Specific Information
Immunogen sequence:
PTAQHLRQSS FEIPDDVPLP AGWEMAKTSS GQRYFLNHID QTTTWQDPRK AMLSQMNVTA PTSPPVQQNM MNSASGPLPD GWEQAMTQDG EIYYINHKNK TTSWLDPRLD PRFAMNQRIS QSAPVKQPPP LAPQSPQGGV MGGSNSNQQQ QMRLQQLQME KERLRLKQQE LLRQAMRNIN PSTANSPKCQ ELALRSQLPT LEQDGGTQNP VSSPGMSQEL RTMTTNSSDP FLNSGTYHSR DESTDSGLSM SSYSVPRTPD DFLNSVDEMD TGDTINQSTL PSQQNRFPDY LEAIPGTNVD LGTLEGDGMN IEGEELMPSL QEALSSDILN DMESVLAATK LDKESFLTWL
Target Information
YAP (Yes-Associated Protein) is a founding member of the WW domain family of proteins. It functions as a transcriptional co-activator and oncogene, transcriptionally activated in squamous cell carcinoma cells. YAP is the critical downstream regulatory target in the Hippo signaling pathway, which controls organ size and tumor suppression by restricting proliferation and promoting apoptosis. It also plays key roles in tissue tension, 3D tissue shape, cell proliferation, cell death, and cell migration. Mutations in the IRF5 gene result in coloboma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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