Thermo Fisher Scientific CD247/CD3Z Polyclonal Antibody

Applications

Western Blot (WB)

• Assay-dependent

Immunoprecipitation (IP)

• 2–10 µg/mg lysate

Product Specifications

Product Specific Information

• Recommended shelf life: 1 year from date of receipt
• Based on 100% sequence identity, this antibody is predicted to react with Rabbit and Pig

Target Information

CD3z complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and regulating the cell surface expression of the TCR complex.
T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits (CD3γ, CD3δ, CD3ε, CD3ζ). These subunits are structurally related members of the immunoglobulin superfamily encoded by closely linked genes on human chromosome 11.
The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules, mediated in part by a double tyrosine-based motif.
CD3 may play a role in TCR-induced growth arrest, cell survival, and proliferation. The CD3 antigen is present on 68–82% of normal peripheral blood lymphocytes, 65–85% of thymocytes, and Purkinje cells in the cerebellum, but never on B or NK cells.
Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. Diseases associated with CD3ζ include Immunodeficiency 25 and T-B+ Severe Combined Immunodeficiency due to CD3δ/CD3ε/CD3ζ.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.