Thermo Fisher Scientific Human Vitamin D3 Receptor, Ligand Binding Domain, GST Tag Recombinant Protein

Applications

• Ligand Binding Assay (LBA)

Tested Dilution: Assay-dependent
Publications: -

Product Specifications

Product Specific Information

Vitamin D3 Receptor, Ligand Binding Domain (VDR-LBD) is a 65.50 kDa recombinant human protein (amino acids 93–427) expressed as a GST-tagged fusion protein in baculovirus-infected insect cells.
VDR-LBD was expressed and purified in the absence of exogenous ligands, making it ideal for ligand binding and coregulator displacement studies.

Protein Form: Recombinant, Ligand Binding Domain

Target Information

Steroid receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone.
The 1,25-dihydroxy-vitamin D3 receptor (VDR) belongs to the superfamily of steroid hormone receptors, including estrogen, progesterone, glucocorticoid, androgen, and thyroid hormone receptors.
Vitamin D modulates calcium and phosphorus homeostasis, bone remodeling, cell growth regulation, and differentiation.
Studies have found VDR in the intestine, bone, kidney, epidermis, and cells of the endocrine immune system.

Repression of T-cell proliferation and interleukin-2 (IL-2) gene expression occurs when VDR binds within the IL-2 enhancer.
Formation of VDR/retinoic X receptor (RXR) heterodimers in the presence of intracellular 1,25(OH)₂D₃ interferes with assembly of nuclear factor of activated T-cells (NFATp)/Fos/Jun/DNA complex and subsequent IL-2 gene transcription.

The corresponding gene for the vitamin D receptor is NR1I1.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.