Thermo Fisher Scientific SREBP1 Monoclonal Antibody (2A4)

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 24 publications 24 publications

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

ChIP assay (ChIP)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Hamster, Human

Published species

Avian, Human, Mouse, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

2A4

Immunogen

Recombinant protein encoding N-terminal amino acids 301-407 (the bHLH/leucine zipper domain) of human SREBP-1 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:SREBP1,uniProtId:P36956-1,ncbiNodeId:9606,antigenRange:301-407,antigenLength:1147,antigenImageFileName:MA5-11685_SREBP1_P36956-1_House_mouse.svg,antigenImageFileNamePDP:MA5-11685_SREBP1_P36956-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein A/G

Storage buffer

PBS, pH 7.4, with 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10984077

Product Specific Information

MA5-11685 targets SREBP-1 in WB applications and shows reactivity with Hamster and Human samples.

The MA5-11685 immunogen is recombinant protein encoding N-terminal amino acids 301-407 (the bHLH/leucine zipper domain) of human SREBP-1.

Target Information

Sterol regulatory element-binding proteins (SREBPs) are transcription factors that are members of the basic helix-loop-helix leucine zipper family of DNA binding proteins. Three isoforms have been identified in mammalian tissues that vary in structure, regulation, and function. SREBP-1a and SREBP-1c (originally cloned as ADD1) are protein products of alternative promoter usage of the SREBP-1 gene. The third isoform is transcribed from a different gene, SREBP-2. SREBPs are present as 120 kDa inactive precursors in the endoplasmic reticulum (ER) membrane. Upon activation, the SREBP protein is translocated to the Golgi and proteolytic cleavage occurs resulting in a mature transcriptionally active 60-78 kDa fragment. In liver and adipose tissues, SREBPs have a significant influence on lipid and cholesterol accumulation by inducing the transcription of genes involved in these processes. While SREBP-1 is thought to be more important in regulating the expression of genes involved in triglyceride synthesis and accumulation, SREBP-2 has been more closely linked to those involved in cholesterol synthesis and accumulation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.