Thermo Fisher Scientific ERCC1 Monoclonal Antibody (OTI1A8), TrueMAB
ERCC1 단백질 인식용 마우스 단클론 항체로, Western blot과 유세포 분석에 최적화됨. 동결건조 형태로 제공되며, 재구성 후 1 mg/mL 농도로 사용 가능. DNA 복구 관련 연구에 적합하며, 휴먼 시료에 반응함.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1A8 |
| Immunogen | Full length human recombinant protein of human ERCC1 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
ERCC1 is a non-catalytic component of a structure-specific DNA repair endonuclease responsible for the 5′ incision during DNA repair.
It functions with SLX4 in the initial step of interstrand cross-link (ICL) repair and participates in processing anaphase bridge-generating DNA structures that can cause cytokinesis failure.
Additionally, ERCC1 is required for homology-directed repair (HDR) of DNA double-strand breaks in conjunction with SLX4. (Source: Uniprot)
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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