Thermo Fisher Scientific CD81/TAPA-1 Recombinant Mouse Monoclonal Antibody (rC81, 3442)
CD81/TAPA-1 단백질을 표적으로 하는 Thermo Fisher Scientific의 재조합 마우스 단일클론 항체입니다. Western blot, IHC, ICC, Flow cytometry 등 다양한 응용에 적합하며, HEK293 세포에서 발현된 IgG1, kappa 형 항체입니다. 연구용으로만 사용 가능합니다.
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- 975-MSM3-P1
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2–4 µg/mL |
| Immunocytochemistry (ICC/IF) | 0.5–1 µg/mL |
| Flow Cytometry (Flow) | 0.5–1 µg/10⁶ cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host/Isotype | Mouse / IgG1, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | rC81, 3442 |
| Immunogen | Recombinant full-length human CD81 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA-fixed): Incubate antibody for 30 minutes at room temperature.
Staining of formalin-fixed tissues requires heating sections in 10 mM Tris Buffer with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes. - Western Blot: Use 1–2 µg/mL for 2 hours at room temperature.
Target Information
CD81 (TAPA-1, target of anti-proliferative antibody-1) is a member of the tetraspanin family expressed on most nucleated cells, especially germinal center B cells. It forms complexes with tetraspanin proteins, integrins, coreceptors, and MHC molecules, influencing adhesion, morphology, activation, proliferation, and differentiation of B and T cells.
In muscle, CD81 promotes cell fusion and myotube maintenance, and acts as a receptor for the hepatitis C virus. Like other tetraspanins (CD9, CD37, CD53, CD63, CD82), CD81 is characterized by four hydrophobic domains mediating signal transduction events that regulate cell development and motility.
CD81 associates with CD19, CD21, Leu 13, and integrins, and antibodies to CD81 exhibit anti-proliferative effects on various lymphoid cell lines. It is localized in a tumor-suppressor gene region and considered a candidate gene for malignancies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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