Thermo Fisher Scientific CD80 (B7-1) Monoclonal Antibody (16-10A1), PE-Cyanine7, eBioscience
마우스 CD80(B7-1)을 인식하는 단클론 항체로, PE-Cyanine7 형광 표지된 유세포분석용 시약입니다. 대식세포, 수지상세포, 활성화 B세포 등에서 발현된 CD80 검출에 적합하며, 488–561 nm 레이저 사용 시 최적의 형광 신호를 제공합니다. 연구용 전용 제품입니다.
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2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD80 (B7-1) Monoclonal Antibody (16-10A1), PE-Cyanine7, eBioscience
Applications and Tested Dilution
- Application: Flow Cytometry (Flow)
- Tested Dilution: 1 µg/test
- View 65 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Mouse, Pig |
| Published Species | Mouse |
| Host / Isotype | Armenian hamster / IgG |
| Recommended Isotype Control | Armenian Hamster IgG Isotype Control (eBio299Arm), PE-Cyanine7, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 16-10A1 |
| Conjugate | PE-Cyanine7 |
| Excitation / Emission Max | 569 / 780 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2573369 |
Available Formats
- Unconjugated
- Alexa Fluor 700
- APC
- Biotin
- Brilliant UV 615 / 737
- Brilliant Violet 480 / 605 / 711 / 786
- eFluor 450
- FITC
- Functional Grade
- PE
- PE-Cyanine5
- PerCP-eFluor 710
- Super Bright 436 / 600 / 645
- Request custom conjugation
Product Specific Information
Description:
The 16-10A1 monoclonal antibody reacts with mouse CD80 (B7-1), a 55 kDa member of the Ig superfamily. CD80 is expressed by macrophages, dendritic cells, and activated B cells. Activated T cells also express this antigen. CD80 binds with high affinity to CD28 and CD152 (CTLA-4) on T cells, facilitating T-B cell communication and activation.
Applications Reported:
Reported for use in flow cytometric analysis.
Applications Tested:
Tested by flow cytometric analysis of stimulated mouse splenocytes. Recommended ≤1 µg per test (in 100 µL final volume). Cell number may range from 10⁵–10⁸ cells/test. Optimize concentration empirically.
Light Sensitivity:
This tandem dye is sensitive to photo-induced oxidation. Protect from light.
Fixation:
Samples can be fixed using IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-54) for up to 3 days at 4°C in the dark with minimal impact on brightness and FRET efficiency. Clone-specific performance should be verified empirically.
Excitation / Emission:
488–561 nm / 775 nm
Compatible Lasers: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD80 (B7-1) and CD86 (B7-2) are ligands for T cell costimulatory receptor CD28 and inhibitory receptor CTLA-4 (CD152). Both are expressed on antigen-presenting cells and are essential for T cell activation. CD80 expression is induced on activated B cells, EBV-transformed B cell lines, Burkitt’s lymphoma cells, T cells, and monocytes.
CD80 provides regulatory signals for T lymphocytes via CD28 and CTLA-4 binding.
Diseases associated with CD80 dysfunction include gallbladder squamous cell carcinoma and myocarditis.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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