Thermo Fisher Scientific CD90.2 (Thy-1.2) Monoclonal Antibody (53-2.1), PE-Cyanine7, eBioscience

Thermo Fisher Scientific CD90.2 (Thy-1.2) Monoclonal Antibody (53-2.1), PE-Cyanine7, eBioscience

Applications

• Flow Cytometry (Flow): Tested at 0.06 µg/test
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Product Specifications

Additional Formats

• Unconjugated
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 615
• eFluor 450
• eFluor 506
• FITC
• NovaFluor Blue 510 / 555 / 610-70S / 660-120S / 760
• NovaFluor Red 710
• NovaFluor Yellow 570 / 660
• PE
• PE-Cyanine5
• PE-eFluor 610
• Super Bright 436 / 600 / 645 / 702
• Custom conjugation available

Product Specific Information

The 53-2.1 monoclonal antibody reacts with mouse CD90.2 (Thy-1.2), a GPI-linked membrane molecule expressed by mouse thymocytes, mature T cells, and neurons in CD90.2-expressing strains (BALB/c, CBA, C3H, C57BL/6, C58, SJL, etc.).
Cells from CD90.1-expressing strains (PL, AKR) do not stain with 53-2.1.
CD90 regulates adhesion and signal transduction in T cells.

Applications Reported: Flow cytometric analysis.
Applications Tested: Flow cytometric analysis of mouse splenocytes at ≤0.06 µg/test (100 µL final volume).
Cell count: 10⁵–10⁸ cells/test (empirical optimization recommended).

Light Sensitivity: Protect from light; tandem dye sensitive to photo-induced oxidation.
Fixation: Samples can be stored in IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-54) for up to 3 days at 4°C in the dark.
Excitation / Emission: 488–561 nm / 775 nm; compatible with Blue, Green, Yellow-Green lasers.
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD90.2 (Thy-1) is a GPI-linked glycoprotein in the Immunoglobulin superfamily.
Expressed in murine T cells, thymocytes, neural cells, Kupffer’s cells, and fibroblasts.
Involved in cell-cell or cell-ligand interactions during synaptogenesis and other brain events.
CD90.2 is an 18 kDa plasma membrane antigen expressed by hematopoietic and neuronal cells, connective tissue, fibroblast and stromal cell lines, and tumor endothelial cell lines.
In mice, CD90.2 is expressed on all thymocytes and peripheral T lymphocytes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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