Thermo Fisher Scientific CD90.2 (Thy-1.2) Monoclonal Antibody (53-2.1), PE-Cyanine7, eBioscience
Mouse CD90.2 (Thy-1.2) 단백질을 인식하는 53-2.1 클론의 Rat IgG2a 단일클론 항체로, PE-Cyanine7 형광으로 결합되어 Flow Cytometry 분석에 최적화됨. 마우스 T 세포 및 신경세포 표지에 사용되며, 높은 특이성과 안정적인 형광 신호 제공.
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Thermo Fisher Scientific CD90.2 (Thy-1.2) Monoclonal Antibody (53-2.1), PE-Cyanine7, eBioscience
Applications
- Flow Cytometry (Flow): Tested at 0.06 µg/test
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Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), PE-Cyanine7, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 53-2.1 |
| Conjugate | PE-Cyanine7 |
| Excitation / Emission Max | 569 / 780 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_469641 |
Additional Formats
- Unconjugated
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 615
- eFluor 450
- eFluor 506
- FITC
- NovaFluor Blue 510 / 555 / 610-70S / 660-120S / 760
- NovaFluor Red 710
- NovaFluor Yellow 570 / 660
- PE
- PE-Cyanine5
- PE-eFluor 610
- Super Bright 436 / 600 / 645 / 702
- Custom conjugation available
Product Specific Information
The 53-2.1 monoclonal antibody reacts with mouse CD90.2 (Thy-1.2), a GPI-linked membrane molecule expressed by mouse thymocytes, mature T cells, and neurons in CD90.2-expressing strains (BALB/c, CBA, C3H, C57BL/6, C58, SJL, etc.).
Cells from CD90.1-expressing strains (PL, AKR) do not stain with 53-2.1.
CD90 regulates adhesion and signal transduction in T cells.
Applications Reported: Flow cytometric analysis.
Applications Tested: Flow cytometric analysis of mouse splenocytes at ≤0.06 µg/test (100 µL final volume).
Cell count: 10⁵–10⁸ cells/test (empirical optimization recommended).
Light Sensitivity: Protect from light; tandem dye sensitive to photo-induced oxidation.
Fixation: Samples can be stored in IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-54) for up to 3 days at 4°C in the dark.
Excitation / Emission: 488–561 nm / 775 nm; compatible with Blue, Green, Yellow-Green lasers.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD90.2 (Thy-1) is a GPI-linked glycoprotein in the Immunoglobulin superfamily.
Expressed in murine T cells, thymocytes, neural cells, Kupffer’s cells, and fibroblasts.
Involved in cell-cell or cell-ligand interactions during synaptogenesis and other brain events.
CD90.2 is an 18 kDa plasma membrane antigen expressed by hematopoietic and neuronal cells, connective tissue, fibroblast and stromal cell lines, and tumor endothelial cell lines.
In mice, CD90.2 is expressed on all thymocytes and peripheral T lymphocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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