Thermo Fisher Scientific H3K4me3 Recombinant Rabbit Monoclonal Antibody (9H28L57), ChIP-Verified
H3K4me3 표적에 특이적인 recombinant rabbit monoclonal 항체로, ChIP 검증 완료. Western blot, ICC/IF, ChIP, Peptide Array 등 다양한 응용에 적합. Human, Mouse, Rat 반응성. 단백질 A 정제, PBS buffer, 0.09% sodium azide 포함.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:4,000 |
| Immunocytochemistry (ICC/IF) | 5 µg/mL |
| ChIP assay (ChIP) | 1–5 µg/million cells |
| Peptide Array (Array) | 1:4,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 9H28L57 |
| Immunogen | Peptide corresponding to human H3K4me3 [aa2–aa9] |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2848245 |
Product Specific Information
This antibody is predicted to react with non-human primate.
Target Information
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3, along with four core histone proteins, binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. Post-translationally, histones are modified in various ways to either directly change chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail of histone H3 protrudes from the nucleosome core and can undergo several post-translational modifications, including methylation or acetylation of lysine and arginine residues and phosphorylation of serine or threonine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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