Thermo Fisher Scientific Phospho-NEDD4L (Ser342) Polyclonal Antibody
Phospho-NEDD4L (Ser342) 폴리클로날 항체로 인간, 마우스, 랫트 시료에 반응. IHC, ICC/IF, ELISA 등 다양한 응용 가능. Protein A로 정제된 고순도 항체로 -20°C에서 안정 보관. 연구용으로 TGF-β 신호 조절 및 단백질 유비퀴틴화 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic phosphopeptide derived from human NEDD4L around the phosphorylation site of Ser342 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
NEDD4L is an E3 ubiquitin-protein ligase that transfers ubiquitin from an E2 enzyme to target substrates. It inhibits TGF-beta signaling by promoting ubiquitination and degradation of SMAD2 and TGFR1. NEDD4L also mediates ubiquitination and internalization of multiple plasma membrane channels such as ENaC, Nav, Kv1.3, EAAT1, and CLC5, as well as degradation of SGK.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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