Thermo Fisher Scientific MIDN Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.04-0.4 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human MIDN. Recombinant protein control fragment (Product #RP-99143). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:MIDN,uniProtId:Q504T8-1,ncbiNodeId:9606,antigenRange:66-164,antigenLength:468,antigenImageFileName:PA5-59825_MIDN_Q504T8-1_Rabbit.svg,antigenImageFileNamePDP:PA5-59825_MIDN_Q504T8-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2643967

Product Specific Information

Immunogen sequence: VPKERLALLH KDTRLSSGKL QEFGVGDGSK LTLVPTVEAG LMSQASRPEQ SVMQALESLT ETQVSDFLSG RSPLTLALRV GDHMMFVQLQ LAAQHAPLQ

Highest antigen sequence identity to the following orthologs: Mouse - 100%, Rat - 70%.

Target Information

Expression of the midnolin gene is developmentally regulated: it is strongly expressed at the mesencephalon (midbrain) of the embryo in day 12.5 (E12.5) mice. The midnolin encodes a protein of 508 amino acids (aa), which contains a Ubiquitin-like domain. The intracellular distribution of the midnolin was studied by using midnolin-green fluorescent protein (GFP) fusion proteins. Midnolin was found to be localized in the nucleus and nucleolus, but not in the cytoplasm. The nucleolar localization signal was determined to be a 28aa peptide located at the C-terminal region of the midnolin. May be involved in regulation of genes related to neurogenesis in the nucleolus.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.