Thermo Fisher Scientific CD279 (PD-1) Monoclonal Antibody (eBioJ105 (J105)), Super Bright 702, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (1.0 µg)/test
• Publications: View 12 publications

Product Specifications

Available Formats

• Unconjugated
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 615 / 661 / 737 / 805
• Brilliant Violet 421 / 480 / 650 / 711 / 786
• PE / PE-Cyanine5 / PE-Cyanine7 / PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436 / 600 / 645 / 780
• Request custom conjugation

Product Specific Information

The J105 monoclonal antibody reacts with human PD-1 (programmed death-1), a 55 kDa member of the CD28 immunoglobulin superfamily. PD-1 contains an immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in peripheral tolerance and autoimmune regulation. PD-1 is expressed mainly on activated T and B lymphocytes. Its ligands, PD-L1 (B7-H1) and PD-L2 (B7-DC), show overlapping functions and are expressed on normal tissues and activated antigen-presenting cells.

Costaining experiments indicate that eBioJ105 recognizes a different epitope than MIH4 (Product # 11-9969-42).

Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-diluted and validated for flow cytometry using stimulated normal human peripheral blood cells. Use at 5 µL (1.0 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

Fluorochrome Information:
Super Bright 702 is a tandem dye excited by the violet laser (405 nm) and emits at 702 nm. Recommended detection filter: 710/50 bandpass. Ensure instrument compatibility.

Staining Recommendations:
When using multiple Super Bright dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.

Light Sensitivity:
Protect from light to prevent photo-induced oxidation.

Fixation:
Samples can be stored in IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days at 4°C (dark conditions) with minimal effect on brightness and FRET efficiency.

Excitation: 405 nm
Emission: 702 nm
Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

T-cell activation is regulated by inhibitory receptors such as PD-1 (PDCD-1), which binds ligands PD-L1 and PD-L2 on antigen-presenting cells (APCs) to suppress immune activation. PD-1 signaling limits immune responses in the absence of danger signals (e.g., LPS). PD-1 knockout mice exhibit hyperactivated immune systems and autoimmune symptoms. Although PD-1 has a predicted molecular weight of 55 kDa, it often migrates at a higher molecular weight in SDS-PAGE.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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