Thermo Fisher Scientific Calprotectin/MRP14/S100A9/Calgranulin B Monoclonal Antibody (47-8D3 (DF3973))
Calprotectin/MRP14/S100A9 단백질을 인식하는 마우스 단클론 항체. IHC, ICC/IF, Flow Cytometry에 사용 가능. 인간 및 랫트 반응성. 단백질 A/G로 정제된 액상 항체로, 보존제 무첨가. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 47-8D3 (DF3973) |
| Immunogen | Human peripheral blood monocyte components |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The S100A8/A9 heterocomplex, also called MRP-8/MRP-14 complex or calprotectin, comprises about 60% of the cytoplasmic protein fraction of circulating polymorphonuclear granulocytes and is also found in monocytes, macrophages, and ileal tissue eosinophils.
Peripheral blood monocytes carry the antigen both extra- and intracellularly, whereas neutrophils carry it only intracellularly.
The S100A8/A9 complex exhibits antibacterial, antifungal, immunomodulating, and antiproliferative activities, and acts as a potent chemotactic factor for neutrophils. Plasma concentrations are elevated in diseases associated with increased neutrophil activity, such as inflammatory bowel disease.
Calprotectin can also be detected in feces due to granulocyte transmigration through the intestinal wall, and elevated levels have been observed in plasma, saliva, gingival crevicular fluid, stools, and synovial fluid during infection and inflammatory conditions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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