Thermo Fisher Scientific DYKDDDDK Tag Polyclonal Antibody
DYKDDDDK(FALG) 태그 단백질 검출용 토끼 폴리클로날 항체. Western blot과 Immunoprecipitation에 적합. 항원 친화 크로마토그래피로 정제된 액상 항체. 단백질 발현, 정제 및 상호작용 연구에 활용 가능. 연구용 한정.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000 |
| Immunoprecipitation (IP) | 1:10–1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide immune sequence is CDYKDDDDK. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 7.4, with 25% glycerol, 0.2% BSA |
| Contains | 0.05% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932598 |
Target Information
DYKDDDDK tag, also known as FLAG tag, is a short peptide sequence commonly used as a protein tag to facilitate protein purification, detection, and localization. The tag consists of eight amino acids: Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys. It can be fused to the N- or C-terminus of a protein of interest, enabling easy identification and isolation using anti-FLAG antibodies.
The DYKDDDDK tag is widely used in molecular biology and biochemistry research, including:
- Protein-protein interaction studies
- Protein expression analysis
- Protein localization studies
The FLAG tag structure is optimized for compatibility with attached proteins; its hydrophilic nature reduces the risk of denaturation or inactivation.
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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