Thermo Fisher Scientific Neural/Glial Antigen 2 (NG2) Monoclonal Antibody (9.2.27), Alexa Fluor 488, eBioscience

Thermo Fisher Scientific Neural/Glial Antigen 2 (NG2) Monoclonal Antibody (9.2.27), Alexa Fluor 488, eBioscience

Applications and Tested Dilution

Product Specifications

Product Specific Information

This 9.2.27 monoclonal antibody reacts with human neural/glial antigen 2 (NG2), also known as melanoma chondroitin sulfate proteoglycan (MCSP).
NG2 consists of a 250 kDa N-linked glycoprotein and a >450 kDa proteoglycan. It is present on >90% of malignant melanomas and some non-melanomic tumors, as well as glioma cells and oligodendrocyte precursor cells.
NG2 is involved in tumor cell proliferation, adhesion, migration, and invasion, and functions as a coreceptor for α4β1 integrin. Expression of NG2 serves as a prognostic marker for disorders such as acral lentiginous melanoma and infantile acute myeloid leukemia.
The 9.2.27 antibody has been reported to suppress melanoma tumor growth.

Applications Reported:

• Flow cytometric analysis
• Immunohistochemical staining of formalin-fixed paraffin embedded tissue (IHC-P)
• Immunocytochemical staining of fixed cells (ICC)

Applications Tested:

• ICC on methanol-fixed A375 cells ≤ 20 µg/mL
• Flow cytometry on A375 cells ≤ 0.125 µg/test
  A test is defined as the antibody amount (µg) required to stain a cell sample in a final volume of 100 µL. Cell number should be empirically determined (10⁵–10⁸ cells/test). Titrate for optimal performance.

Excitation: 488 nm
Emission: 519 nm
Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered

Target Information

Human melanoma-associated chondroitin sulfate proteoglycan (CSPG4) stabilizes cell-substratum interactions during early melanoma cell spreading on endothelial basement membranes. CSPG4 is an integral membrane proteoglycan expressed by human malignant melanoma cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지