Thermo Fisher Scientific NEFM Monoclonal Antibody (OTI5B7), TrueMAB
인간 NEFM 단백질을 인식하는 마우스 모노클로날 항체로, Western blot 및 IHC(P)에서 검증됨. 고순도 친화 크로마토그래피 정제, 무보존제 리오필라이즈드 형태. 신경세포 내 중간 필라멘트 연구에 적합.
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Thermo Fisher Scientific NEFM Monoclonal Antibody (OTI5B7), TrueMAB
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI5B7 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 413–773 of human NEFM produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
Neurofilaments (NFs) are intermediate filaments expressed almost exclusively in neuronal cells, especially in large axons. They consist of three major subunits—NF-M, NF-H, and NF-L—with structural similarity in their coiled-coil core domains but differences in terminal regions.
NF-M tail-mediated interactions are essential for axon size and cytoskeletal architecture, regulated by phosphorylation and O-GlcNAcylation.
Antibodies against neurofilament subunits serve as reliable neuronal markers due to their abundance, neuron-specific expression, and biochemical stability.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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