Thermo Fisher Scientific PYCRL Monoclonal Antibody (OTI3B9), TrueMAB
Human PYCRL 단백질을 인식하는 Mouse IgG2a 단일클론 항체로, WB, IHC, ICC/IF, Flow Cytometry에 사용 가능. 동결건조 형태이며 PBS buffer에 trehalose 포함. 연구용으로 proline 생합성 관련 PYCRL 단백질 분석에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3B9 |
| Immunogen | Full length human recombinant protein of human PYCRL produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of about 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
PYCRL encodes a protein belonging to the pyrroline-5-carboxylate reductase family of enzymes. This family catalyzes the final step in proline biosynthesis, converting pyrroline-5-carboxylate to proline. Glutamate and ornithine serve as precursors in proline synthesis. The PYCRL protein is a cytoplasmic enzyme involved in proline biosynthesis from ornithine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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