Thermo Fisher Scientific CD27 Monoclonal Antibody (O323)

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 2–10 µg/mL

Flow Cytometry (Flow)

• Tested Dilution: 0.5–5 µg/10^6 cells

Product Specifications

Product Specific Information

• Positive control: Tonsil
• Cellular location: Membrane

Target Information

CD27 is a 50 kDa member of the tumor necrosis factor (TNF) receptor superfamily, which includes CD40 and CD30. Members of this family regulate cell proliferation and death. CD27 and its ligand CD70 are primarily expressed on lymphocytes. High CD27 expression depends on antigen receptor ligation, while CD70 expression requires additional co-stimulatory or pro-inflammatory signals.

CD27 forms a disulfide-linked homodimer on mature thymocytes, peripheral blood T cells, and a subset of B cells. T cell activation via the TCR-CD3 complex upregulates CD27 expression and induces release of its soluble form (sCD27, 28–32 kDa), detectable in plasma, urine, or spinal fluid. Soluble CD27 serves as a prognostic marker for acute and chronic B cell malignancies.

RgpA, a cysteine proteinase, can degrade CD27, counteracting T cell activation mediated by CD27–CD70 interaction. CD27-binding protein (SIVA), a proapoptotic protein, binds to CD27 and is involved in apoptosis induced by this receptor. CD27 dysfunction is associated with Lymphoproliferative Syndrome 2 and Autosomal Recessive Lymphoproliferative Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.