Thermo Fisher Scientific YBX1/Y-box Binding Protein 1/YB-1 Monoclonal Antibody (YBX1, 2430)

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1–2 µg/mL

Peptide Array (Array)

• Tested Dilution: Assay-dependent

Product Specifications

Product Specific Information

• For immunohistochemistry (PFA-fixed): incubate antibody for 30 minutes at room temperature.
• Staining of formalin-fixed tissues requires heating sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.

Target Information

YBX1 binds to splice sites in pre-mRNA and regulates splice site selection. It stabilizes cytoplasmic mRNA and modulates translation by influencing mRNA–eukaryotic initiation factor interactions.
This protein also binds promoters containing a Y-box (5’-CTGATTGGCCAA-3’), such as HLA class II genes, and regulates transcription of numerous genes.
YBX1 promotes separation of mismatched or cisplatin-modified DNA strands, exhibits endonucleolytic activity introducing nicks or breaks into double-stranded DNA (in vitro), and may play a role in DNA repair.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.