Thermo Fisher Scientific Urokinase Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50

-

Immunohistochemistry (Frozen) (IHC (F))

1:50

-

ELISA (ELISA)

1:5,000

-

Product Specifications

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Purified native HMW-tcuPA and LMW-scuPA (mixture 50/50), isolated from human urine.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Storage conditions

4° C

Shipping conditions

Wet ice

RRID

AB_1088179

Product Specific Information

This antibody has been pre-absorbed with immobilized total human serum proteins to remove non-specific antibodies. This antibody is known to bind human HMW-scuPA (54 kDa), HMW-tsuPA (52 kDa), and LMW-scuPA (33 kDa), completely inhibiting the ability of Urokinase to activate plasminogen.

Reconstitute with 1 mL of distilled water and add preservative if preferred.

Target Information

This gene encodes a serine protease involved in degradation of the extracellular matrix and possibly tumor cell migration and proliferation. A specific polymorphism in this gene may be associated with late-onset Alzheimer`s disease and also with decreased affinity for fibrin-binding. This protein converts plasminogen to plasmin by specific cleavage of an Arg-Val bond in plasminogen. Plasmin in turn cleaves this protein at a Lys-Ile bond to form a two-chain derivative in which a single disulfide bond connects the amino-terminal A-chain to the catalytically active, carboxy-terminal B-chain. This two-chain derivative is also called HMW-uPA (high molecular weight uPA). HMW-uPA can be further processed into LMW-uPA (low molecular weight uPA) by cleavage of chain A into a short chain A (A1) and an amino-terminal fragment. LMW-uPA is proteolytically active but does not bind to the uPA receptor. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.