Thermo Fisher Scientific CD25 Monoclonal Antibody (PC61.5), Alexa Fluor 700, eBioscience
마우스 CD25(IL-2Rα)를 인식하는 PC61.5 클론의 Alexa Fluor 700 결합 단일클론 항체. Flow cytometry에 최적화되어 있으며 0.25 µg/test로 사용. 633 nm 레이저로 여기, 723 nm에서 방출. 면역세포 활성 및 분화 연구에 적합.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.25 µg/test | View 90 publications |
| Neutralization (Neu) | - | View 2 publications |
| Functional Assay (Functional) | - | View 2 publications |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG1, lambda |
| Recommended Isotype Control | Rat IgG1 kappa Isotype Control (eBRG1), Alexa Fluor™ 700, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | PC61.5 |
| Conjugate | Alexa Fluor™ 700 |
| Excitation/Emission Max | 696 / 719 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_891424 |
Available Formats
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 532
- Alexa Fluor 561
- Alexa Fluor 647
- Alexa Fluor 660
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 563 / 661 / 737 / 805
- Brilliant Violet 421 / 650 / 711 / 786
- eFluor 450 / 506
- FITC
- Functional Grade
- NovaFluor Blue 585 / 610-70S / 660-120S / 760
- Pacific Orange
- PE / PE-Cyanine5 / PE-Cyanine5.5 / PE-Cyanine7 / PE-eFluor 610
- PerCP-Cyanine5.5 / PerCP-eFluor 710
- Super Bright 436 / 600 / 645 / 702 / 780
- Request custom conjugation
Product Specific Information
The PC61.5 antibody reacts with mouse CD25, the 55 kDa interleukin-2 receptor alpha chain (IL-2Rα). CD25 is expressed by early progenitors of the T and B lineage and activated mature T and B lymphocytes.
By itself, CD25 binds IL-2 with low affinity, but associates with CD122 and CD132 to form a high-affinity IL-2 receptor. Binding of IL-2 to both classes of IL-2 receptor is inhibited by PC61.5.
CD25 plays a role in lymphocyte differentiation and activation/proliferation.
Applications Reported: Flow cytometric analysis.
Applications Tested: Mouse splenocytes, ≤ 0.25 µg/test (100 µL final volume).
Cell number: 10⁵–10⁸ cells/test.
Careful titration recommended for optimal performance.
Fluorochrome Details:
Alexa Fluor™ 700 emits at 723 nm and is excited with a red laser (633 nm).
Recommended filters: 685 LP mirror and 710/20 filter.
Excitation: 633–647 nm; Emission: 723 nm; Laser: Red.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD25 (IL2 receptor alpha chain / IL2RA) is a cytokine receptor involved in T and B lymphocyte proliferation.
The IL2 receptor complex (IL2RA, IL2RB, IL2RG) forms a high-affinity receptor; IL2RA homodimers form low-affinity receptors.
IL2 is produced mainly by mature T cells and regulates growth, differentiation, and cell death.
Mutations in IL2RA are associated with interleukin 2 receptor alpha deficiency and immune dysregulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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