Thermo Fisher Scientific p27 Kip1 Polyclonal Antibody
Thermo Fisher Scientific의 p27 Kip1 Polyclonal Antibody는 인간 및 마우스 반응성을 가진 Rabbit IgG 항체입니다. Western blot, IHC, ICC/IF 등 다양한 응용에 적합하며, 고순도 항원 친화 크로마토그래피로 정제되었습니다. 세포주기 조절 단백질 연구에 이상적입니다.
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific p27 Kip1 Polyclonal Antibody
Thermo Fisher Scientific p27 Kip1 Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | View 1 publication |
| Immunohistochemistry (IHC) | – | View 3 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | – |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:1,000 | – |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | View 2 publications |
| Immunoprecipitation (IP) | 1:100–1:500 | – |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 55–198 of Human P27 Kip1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.08 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol, 1% BSA |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2544664 |
Product Specific Information
- Recommended positive controls: HeLa, MCF-7, MDA-MB-231, Neuro 2A, C8D30, NIH-3T3, Raw264.7, C2C12
- Predicted reactivity: Mouse (80%), Dog (87%), Cat (87%), Pig (84%), Rhesus Monkey (98%), Bovine (84%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
p27 Kip1 functions as a negative regulator of G1 phase expression and may mediate TGFβ-induced G1 arrest. It is a crucial regulator of cell cycle progression and inhibits cyclin E- and cyclin A-CDK2 complexes. p27 Kip1 interacts with cyclin D-CDK4 complexes, contributing to their assembly, stability, and activation modulation. Depending on phosphorylation state and stoichiometry, p27 Kip1 can act as either an inhibitor or activator of cyclin D-CDK4 complexes. This protein (~27 kDa) undergoes CDK-dependent phosphorylation and ubiquitination by SCF complexes, enabling the transition from quiescence to proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
(이미지 본문에서 제거됨, 하단에 배치)
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.