Thermo Fisher Scientific STAT4 Monoclonal Antibody (OTI1G9), TrueMAB
STAT4 단백질을 인식하는 Mouse IgG1 단일클론 항체로, Western blot, ICC/IF, Flow cytometry에 사용 가능. IL-12 및 IFN-γ 신호 연구에 적합하며, 고순도 정제된 액상 형태로 안정적 저장 가능.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1G9 |
| Immunogen | Full length human recombinant protein of human STAT4 produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Additional Formats
Target Information
STAT4 was originally identified using degenerate primers complementary to sequences encoding conserved regions of other STAT proteins. The STAT4 protein is most similar to STAT1 (52%) and STAT3 (47%). Functionally, STAT4 can be tyrosine phosphorylated by Jak1 or Jak2, forming homodimers and heterodimers with other STAT family members. Tyrosine-phosphorylated STAT4 binds the IFN-gamma activated site (GAS), and serine phosphorylation is required for maximal transcriptional activity.
STAT4 expression is restricted to the thymus, spleen, and testis. It is activated by interleukin 12 (IL-12), which is essential for T-cell independent induction of IFN-gamma and development of Th1 response against intracellular pathogens. STAT4-deficient mice exhibit impaired IL-12-mediated Th1 cell development and enhanced Th2 differentiation. In mouse models, IFN-α/β activation of STAT4 is required for IFN-γ production during viral infection.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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