Takara Lentivirus titration by p24 ELISA

The Lenti-X p24 Rapid Titer Kit (Single Wash) and the Lenti-X p24 Rapid Titer Kit are designed for the rapid determination of lentiviral supernatant titers by p24 ELISA. (See the workflow comparison for both the kits.)

Why do you need to know your titer?

• Confirm the success of your packaging reaction to avoid wasting time with your experiments
• Perform consistent experiments
• Ensure sufficient MOI so that 100% of your cells are transduced
• Control copy number and hence expression levels

The Lenti-X p24 Rapid Titer Kit (Single Wash) and the Lenti-X p24 Rapid Titer Kit are designed for the rapid determination of lentiviral supernatant titers by p24 ELISA.

Why do you need to know your titer?

• Confirm the success of your packaging reaction to avoid wasting time with your experiments
• Perform consistent experiments
• Ensure sufficient MOI so that 100% of your cells are transduced
• Control copy number and hence expression levels

What is p24?

Recombinant lentiviruses generated for research use contain major structural proteins, including envelope proteins, matrix proteins, and virus core proteins. The gag gene encodes the viral capsid protein (p24), the nucleocapsid proteins (p6 and p7), and a matrix protein (p17) (see data images by expanding the Details tab in the product table). The Lenti-X p24 Rapid Titer Kit specifically measures the amount of p24 capsid protein present in your viral supernatant. The level of p24 correlates directly with virus titer.

More Less