Thermo Fisher Scientific GST Polyclonal Antibody, Rhodamine
Rhodamine으로 표지된 GST 폴리클로날 항체로 Western blot, ICC/IF, ELISA, IP 등 다양한 응용에 적합. Glutathione-S-Transferase(S. japonicum) 면역원 사용. 염색 효율이 높고, 573/591 nm에서 형광 검출 가능. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:20,000 |
| Immunocytochemistry (ICC/IF) | 1:500–1:2,500 |
| ELISA | >1:20,000 |
| Immunoprecipitation (IP) | >1:10,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host/Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Glutathione-S-Transferase (Schistosoma japonicum) |
| Conjugate | Rhodamine |
| Excitation/Emission Max | 573/591 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10 mg/mL BSA |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, as well as purified and partially purified Glutathione-S-Transferase (Schistosoma japonicum). Cross-reactivity against Glutathione-S-Transferase from other sources may occur but has not been specifically determined.
Target Information
GST (Glutathione S-transferase) is a 26 kDa protein found in both eukaryotes and prokaryotes, catalyzing various reactions. It is used in the GST gene fusion system, allowing purification of GST-fusion proteins due to their high affinity for glutathione. Fusion proteins are valuable for studying direct protein–protein interactions. The GST fusion system has been introduced into numerous expression vectors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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