Thermo Fisher Scientific VGLL2 Polyclonal Antibody
VGLL2 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot 및 Immunocytochemistry에 사용 가능. 인간, 마우스, 랫트 반응성. 합성 펩타이드 면역원 기반으로 제작되었으며, 액상 형태로 제공. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human VGLL2 (Accession Q8N8G2), corresponding to amino acid residues S116–H166 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | –20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2850197 |
Product Specific Information
This antibody detects endogenous levels of total VGLL2.
Target Information
VGLL2 is a key regulator in Drosophila wing development, acting via interaction with scalloped, a homolog of vertebrate TEF proteins. In humans, VGLL2 expression is detected in skeletal muscle. During mouse embryogenesis, it is expressed in differentiating somites and branchial arches.
VITO1, a clone of VGLL2, contains a scalloped interaction domain (SID) and is highly expressed in differentiated myotubes of the mouse C2C12 cell line. During differentiation, VGLL2 mRNA levels increase and its protein translocates from the cytoplasm to the nucleus. Suppression of VITO1 expression by siRNA attenuates muscle cell differentiation, indicating VGLL2’s critical role in myogenic development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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