Thermo Fisher Scientific RT1.Bu Monoclonal Antibody (OX-3), FITC
Anti-rat RT1.Bu 단백질을 인식하는 OX-3 클론의 FITC 결합 단일클론 항체로, Flow Cytometry에 적합합니다. Lewis, Wistar, AO 등 특정 rat strain에서 Ia 항원을 검출하며, mouse MHC haplotype b 및 s에도 교차 반응합니다. 연구용으로만 사용 가능합니다.
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Thermo Fisher Scientific RT1.Bu Monoclonal Antibody (OX-3), FITC
Applications
- Flow Cytometry (Flow)
Tested Dilution: 0.2 µg/1x10^6 cells
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OX-3 |
| Immunogen | Rat thymocyte membrane glycoprotein |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS with 4–5 mg/mL BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | 4°C (short term), -20°C (long term), avoid freeze/thaw cycles, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2538806 |
Additional Formats
- Unconjugated
- Biotin
- PE
- Custom conjugation available
Product Specific Information
Anti-rat RT1.Bu monoclonal antibody recognizes a polymorphic determinant on rat Ia present on Lewis, Wistar, and AO rat strains (although expressed at lower levels in AO rats) but not BN, DA, or PVG/c strains.
This antibody cross-reacts with mouse strains (MHC haplotypes b and s), and analysis of recombinant mouse strains shows that the determinant maps to the I-A region and correlates with mouse Ia specificity.
Recognizes Ia antigens on approximately 18% of thymocytes but does not significantly label most peripheral T lymphocytes.
Useful for distinguishing Ia-positive cells from different rat strains and identifying donor-origin cells in bone marrow reconstituted radiation chimeras.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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