Thermo Fisher Scientific RT1.Bu Monoclonal Antibody (OX-3), FITC

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

-

Product Specifications

Species Reactivity

Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

OX-3

Immunogen

Rat thymocyte membrane glycoprotein if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:RT1.Bu,uniProtId:P20037-1,ncbiNodeId:10114,antigenRange:1-256,antigenLength:256,antigenImageFileName:MA5-17416_RT1.Bu_P20037-1_House_mouse.svg,antigenImageFileNamePDP:MA5-17416_RT1.Bu_P20037-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

FITC FITC FITC

View additional formats

• Unconjugated
• Biotin
• PE
• Request custom conjugation

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

0.1 mg/mL

Purification

Protein G

Storage buffer

PBS with 4-5mg/mL BSA

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2538806

Product Specific Information

Anti-rat RT1.Bu monoclonal antibody recognizes a polymorphic determinant on rat Ia present on Lewis, Wistar, and AO rat strains (although it is apparently expressed in lower amounts in AO rats) but not BN, DA, or PVG/c strains. This antibody also cross-reacts with mouse strains (MHC haplotypes b and s) and analysis of recombinant mouse strains shows that the determinant maps to the I-A region and correlates with mouse Ia specificity 9. Recognizes Ia antigens on approximately 18% of thymocytes but does not significantly label the majority of peripheral T lymphocytes. Thus, this antibody is particularly useful for distinguishing Ia positive cells from different rat strains and has been used in the recognition of cells of donor origin in bone marrow reconstituted radiation chimeras.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.