Thermo Fisher Scientific CDK4 Monoclonal Antibody (DCS-31.2)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

-

Immunoprecipitation (IP)

Assay-dependent

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

DCS-31.2

Immunogen

Proprietary Immunogen.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

purified

Storage buffer

PBS

Contains

0.08% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_929804

Product Specific Information

Mouse anti CDK4 antibody clone DCS31.2 recognizes cyclin dependent kinase 4 (Cdk4), a ~34 kDa protein involved in the regulation of cell cycle progression between G1 and S.Mouse anti CDK4 antibody clone DCS31.2 does not cross react with other cdk`s and does not co-precipitate the D type cyclins bound to cdk4.

Target Information

CDK4/cyclin D3 serine/threonine kinase is dimeric protein complex. CDK4 is a member of the cyclin-dependent protein kinase family and is involved in the control of cell proliferation during the G1 phase of cell cycle. CDK4 forms a complex with the D-type cyclins and is inhibited by p16 (cyclin-dependent kinase inhibitor-2). CDK4 can mediate phosphorylation of the C-terminal region of Rb protein leading to an active transcriptional repression of E2F complex. CDC37 and HSP90 can preferentially associate with the fraction of CDK4 not bound to D-type cyclins. SMAD3 is a major physiologic substrate of the G1 cyclin-dependent kinases CDK4 and CDK2. Although CDK6 and CDK4 can both phosphorylate multiple residues in the Rb protein, they do so with different residue selectivity in vitro; CDK6 phosphorylates Thr821 while CDK4 phosphorylates Thr826 on Rb protein.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.