Thermo Fisher Scientific CDK4 Monoclonal Antibody (DCS-31.2)

Applications

Product Specifications

Product Specific Information

Mouse anti-CDK4 antibody clone DCS-31.2 recognizes cyclin-dependent kinase 4 (Cdk4), a ~34 kDa protein involved in regulating cell cycle progression between G1 and S phases.
This antibody does not cross-react with other CDKs and does not co-precipitate D-type cyclins bound to CDK4.

Target Information

CDK4/cyclin D3 serine/threonine kinase is a dimeric protein complex. CDK4, a member of the cyclin-dependent protein kinase family, controls cell proliferation during the G1 phase of the cell cycle.
It forms a complex with D-type cyclins and is inhibited by p16 (cyclin-dependent kinase inhibitor-2). CDK4 mediates phosphorylation of the C-terminal region of Rb protein, leading to transcriptional repression of the E2F complex.
CDC37 and HSP90 preferentially associate with CDK4 not bound to D-type cyclins. SMAD3 is a major physiologic substrate of G1 cyclin-dependent kinases CDK4 and CDK2.
Although CDK6 and CDK4 both phosphorylate multiple residues in the Rb protein, they exhibit different residue selectivity in vitro: CDK6 phosphorylates Thr821, while CDK4 phosphorylates Thr826.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.