Thermo Fisher Scientific Vimentin Polyclonal Antibody
Rabbit polyclonal antibody targeting human vimentin for WB applications. Recognizes human, mouse, and rat species. High purity (>95%) and antigen affinity purified. Supplied in liquid form with glycerol for stability. Suitable for research use only.
- 판매단위
- pk
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Applications
- Western Blot (WB): 1:500–1:1,000 dilution
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids 111–160 of Human Vimentin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS (pH 7.2) with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term; for long-term storage, store at −20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2803318 |
Product Specific Information
- Purity is >95% by SDS-PAGE.
Target Information
Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is involved in intracellular transport of proteins between the nucleus and plasma membrane. Unlike other IFP proteins, vimentin is expressed with desmin during early cellular development and is later replaced by tissue-specific IFPs.
Vimentin also participates in steroid synthesis by acting as a storage network for cholesterol-containing lipid droplets. Phosphorylation of vimentin by protein kinase induces filament breakdown and cytoskeletal rearrangements that facilitate cholesterol transport to mitochondria, enhancing steroid biosynthesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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