Thermo Fisher Scientific ATG10 Polyclonal Antibody

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant Protein corresonding to Human ATG10. Recombinant protein control fragment (Product #RP-102958). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:ATG10,uniProtId:Q9H0Y0-1,ncbiNodeId:9606,antigenRange:26-138,antigenLength:220,antigenImageFileName:PA5-111196_ATG10_Q9H0Y0-1_Rabbit.svg,antigenImageFileNamePDP:PA5-111196_ATG10_Q9H0Y0-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2856606

Product Specific Information

Immunogen sequence: IGDSWEWRPS KDCSDGYMCK IHFQIKNGSV MSHLGASTHG QTCLPMEEAF ELPLDDCEVI ETAAASEVIK YEYHVLYSCS YQVPVLYFRA SFLDGRPLTL KDIWEGVHEC YKM

Target Information

ATG10 is involved in autophagy which is a process for the bulk degradation of cytosolic compartments by lysosomes/vacuoles. The formation of autophagosomes involves a dynamic rearrangement of the membrane for which two ubiquitin-like modifications (the conjugation of APG12p and the modification of a soluble form of MAP-LC3 to a membrane-bound form) are essential. APG10 is an E2-like enzyme involved in two ubiquitin-like modifications essential for autophagosome formation, ATG12 -ATG5 conjugation and modification of a soluble form of MAP-LC3 (MAP1LC3A), a homolog of yeast Apg8, to a membrane-bound form. The protein ATG10 is ubiquitously expressed and the human APG10L gene is localized on chromosome 5q14.2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.