Thermo Fisher Scientific ATG12-ATG5 Recombinant Rabbit Monoclonal Antibody (20H20L20)

Applications

• Western Blot (WB): 1–2 µg/mL

Product Specifications

Product Specific Information

This antibody is predicted to react with Monkey, Pig, Cat, and Mouse.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages of recombinant rabbit monoclonal antibodies include:

• Better specificity and sensitivity
• Lot-to-lot consistency
• Animal origin-free formulations
• Broader immunoreactivity to diverse targets due to the larger rabbit immune repertoire

Target Information

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway, is important for normal growth control and may be defective in tumor cells. It is involved in preserving cellular nutrients under starvation and normal turnover of cytosolic components.
This process is negatively regulated by TOR (Target of Rapamycin) through phosphorylation of autophagy protein APG1.
ATG12, a member of the autophagy protein family, forms a conjugate with ATG5 that has ubiquitin-protein ligase (E3)-like activity for protein lipidation in autophagy.
This conjugate also associates with innate immune response proteins such as RIG-I and VISA (IPS-1), inhibiting type I interferon production and permitting viral replication in host cells.
ATG12 interacts with ATG10 in human embryonic kidney cells in the presence of ATG7. At least two isoforms of ATG12 are known.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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