Thermo Fisher Scientific SAP97 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:5,000

View 16 publications 16 publications

Immunohistochemistry (IHC)

-

View 5 publications 5 publications

Immunohistochemistry (Frozen) (IHC (F))

1:1,000

-

Immunohistochemistry - Free Floating (IHC (Free))

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:200 - 1:250

View 6 publications 6 publications

Immunoprecipitation (IP)

Assay-dependent

View 5 publications 5 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues V(115) L P S E R I S P Q V P N E V L G P E(133) of rat SAP97. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:SAP97,uniProtId:Q62696-1,ncbiNodeId:10114,antigenRange:115-133,antigenLength:911,antigenImageFileName:PA1-741_SAP97_Q62696-1_Rabbit.svg,antigenImageFileNamePDP:PA1-741_SAP97_Q62696-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Purification

Ammonium sulfate precipitation

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2092020

Product Specific Information

PA1-741 detects Synapse-Associated Protein 97 (SAP97) from human, rat and mouse tissues. No cross-reactivity to other proteins, including synapse-associated protein family members, has been observed.

PA1-741 has been successfully used in Western blot, immunofluorescence, immunocytochemistry, immunoprecipitation, and immunohistochemistry procedures. By Western blot, this antibody detects an ~140 kDa protein representing SAP97 from rat brain extract. Immunohistochemical staining of SAP97 in rat hippocampus with PA1-741 results in intense neuronal cell body and dendrite staining.

The PA1-741 immunizing peptide corresponds to amino acids 115-133 from rat SAP97. This sequence is 84% and 74% conserved in mouse and human SAP97, respectively.

PA1-741 can be used with blocking peptide PEP-055.

Target Information

This gene encodes a multi-domain scaffolding protein that is required for normal development. This protein may have a role in septate junction formation, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene, but the full-length nature of some of the variants is not known.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.