Thermo Fisher Scientific SAP97 Polyclonal Antibody
SAP97 단백질을 인식하는 토끼 폴리클로날 항체로, 인간, 생쥐, 랫트 시료에 반응합니다. WB, IHC, ICC/IF, IP 등 다양한 응용에서 사용 가능하며, 약 140 kDa 단백질을 검출합니다. PBS 완충액에 0.05% sodium azide가 포함되어 있으며 -20°C에서 보관합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:5,000 | View 16 publications |
| Immunohistochemistry (IHC) | – | View 5 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:1,000 | – |
| Immunohistochemistry – Free Floating (IHC (Free)) | – | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1:200–1:250 | View 6 publications |
| Immunoprecipitation (IP) | Assay-dependent | View 5 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to residues V(115) L P S E R I S P Q V P N E V L G P E(133) of rat SAP97 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not determined |
| Purification | Ammonium sulfate precipitation |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2092020 |
Product Specific Information
PA1-741 detects Synapse-Associated Protein 97 (SAP97) from human, rat, and mouse tissues.
No cross-reactivity to other proteins, including synapse-associated protein family members, has been observed.
PA1-741 has been successfully used in Western blot, immunofluorescence, immunocytochemistry, immunoprecipitation, and immunohistochemistry procedures.
By Western blot, this antibody detects an approximately 140 kDa protein representing SAP97 from rat brain extract.
Immunohistochemical staining of SAP97 in rat hippocampus with PA1-741 results in intense neuronal cell body and dendrite staining.
The PA1-741 immunizing peptide corresponds to amino acids 115–133 from rat SAP97.
This sequence is 84% and 74% conserved in mouse and human SAP97, respectively.
PA1-741 can be used with blocking peptide PEP-055.
Target Information
This gene encodes a multi-domain scaffolding protein required for normal development.
It may play roles in septate junction formation, signal transduction, cell proliferation, synaptogenesis, and lymphocyte activation.
Several alternatively spliced transcript variants encoding different isoforms have been described, though the full-length nature of some variants is not known.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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