Thermo Fisher Scientific STAT4 Monoclonal Antibody (OTI4F9), TrueMAB
인간 STAT4 단백질을 인식하는 마우스 모노클로날 항체로, Western blot, IHC, Flow Cytometry에 적합. TrueMAB 포맷으로 높은 특이성과 재현성 제공. 연구용으로 STAT4 신호전달 및 면역 반응 분석에 활용 가능.
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Thermo Fisher Scientific STAT4 Monoclonal Antibody (OTI4F9), TrueMAB
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI4F9 |
| Immunogen | Full length human recombinant protein of human STAT4 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.28 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
STAT4 was originally identified using degenerate primers complementary to sequences encoding conserved regions of other STAT proteins. The STAT4 protein shows similarity to STAT1 (52%) and STAT3 (47%). It can be tyrosine phosphorylated by Jak1 or Jak2 and forms homo- and heterodimers with related STAT family members.
Tyrosine phosphorylated STAT4 binds the IFN-gamma activated site (GAS), and serine phosphorylation is required for maximal transcriptional activity. STAT4 expression is restricted to the thymus, spleen, and testis. It is activated by interleukin 12 (IL-12), which is essential for IFN-gamma induction and Th1 response development, aiding host defense against intracellular pathogens. STAT4-deficient mice show impaired Th1 development and enhanced Th2 differentiation. In viral infection, IFN-a/b activation of STAT4 is required for IFN-gamma production.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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배송/결제/교환/반품 안내
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