Thermo Fisher Scientific Podocin Recombinant Rabbit Monoclonal Antibody (2O10G3)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

2O10G3

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 284-383 of human NPHS2 (Q9NP85). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Podocin,uniProtId:Q9NP85-1,ncbiNodeId:9606,antigenRange:284-383,antigenLength:383,antigenImageFileName:MA5-42776_Podocin_Q9NP85-1_Rabbit.svg,antigenImageFileNamePDP:MA5-42776_Podocin_Q9NP85-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.2 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 0.05% BSA, 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2911917

Product Specific Information

Positive test controls include: HeLa, HT-29, 293T.

Immunogen sequence: AQRQAKVRMI AAEAEKAASE SLRMAAEILS GTPAAVQLRY LHTLQSLSTE KPSTVVLPLP FDLLNCLSSP SNRTQGSLPF PSPSKPVEPL NPKKKDSPML

Target Information

The pathological conditions of SRN1 correlate well with mutations at the NPHS2 gene, where expression of a protein known as Podocin occurs. Abnormal or inefficient signaling through Podocin protein-dependent networks contributes to the development of podocyte dysfunction and proteinuria. The human NPHS2 gene maps to chromosome 1q25-q31 and encodes a 383 amino acid protein. Podocin is an integral membrane protein that appears to fold into a hairpin-like structure with intracellular amino- and carboxy-termini. Transmembrane and cytoplasmic portions of Podocin share homology to the corresponding regions of the stomatin family proteins. Expression of high-order oligomers of Podocin in glomerular podocytes may reflect a scaffolding function that influences proper function of the glomerular filtration barrier, which is necessary for renal stability.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.