Thermo Fisher Scientific ZAK Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 100-312 of human ZAK (NP_5984071) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:ZAK,uniProtId:Q9NYL2-1,ncbiNodeId:9606,antigenRange:100-312,antigenLength:800,antigenImageFileName:PA5-89875_ZAK_Q9NYL2-1_Rabbit.svg,antigenImageFileNamePDP:PA5-89875_ZAK_Q9NYL2-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.76 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.01% thimerosal

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2805817

Product Specific Information

Immunogen sequence: EEMDMDHIMT WATDVAKGMH YLHMEAPVKV IHRDLKSRNV VIAADGVLKI CDFGASRFHN HTTHMSLVGT FPWMAPEVIQ SLPVSETCDT YSYGVVLWEM LTREVPFKGL EGLQVAWLVV EKNERLTIPS SCPRSFAELL HQCWEADAKK RPSFKQIISI LESMSNDTSL PDKCNSFLHN KAEWRCEIEA TLERLKKLER DLSFKEQELK ERE; Positive Samples: Mouse heart, Mouse brain, Mouse skeletal muscle, Rat heart, Rat skeletal muscle; Cellular Location: Cytoplasm, Nucleus

Target Information

ZAK is a leucine zipper-containing mitogen-activated protein related to the MLKs in both sequence and function. Like the MLKs and other MAP3Ks, ZAK activates MAP2Ks. ZAK is a member of the MAPKKK family of signal transduction molecules and encodes a protein with an N-terminal kinase catalytic domain, followed by a leucine zipper motif and a sterile-alpha motif. This magnesium-binding protein forms homodimers and is located in the cytoplasm. The protein mediates gamma radiation signaling leading to cell cycle arrest and activity of this protein plays a role in cell cycle checkpoint regulation in cells. The protein also has pro-apoptotic activity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.