Thermo Fisher Scientific OCT4 Monoclonal Antibody (9B7)
OCT4 단백질을 인식하는 마우스 단클론 항체로 인간 및 마우스 시료에 반응합니다. Western blot, ICC/IF, Flow Cytometry 등 다양한 응용에 사용 가능하며, 단백질 A로 정제된 액상 형태입니다. 배아 줄기세포 연구 및 iPS 세포 생성 연구에 적합합니다.
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Thermo Fisher Scientific OCT4 Monoclonal Antibody (9B7)
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:5,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | – | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL | View 9 publications |
| Flow Cytometry (Flow) | 3–5 µg/1×10⁶ cells | View 3 publications |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 9B7 |
| Immunogen | Full-length human recombinant protein of POU5F1 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with 1 mg/mL BSA, 30% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2536771 |
Additional Formats
Product Specific Information
MA1-104 detects Oct4/POU5F1 in human embryonic carcinoma samples, but not in adult samples such as HeLa cells.
This antibody does not show reactivity in mouse embryonic cell lines (e.g., F9 embryonic carcinoma cells).
It has been successfully used in FACS, IF, and WB applications.
By Western blot, it detects a band at 40 kDa in human NCCIT and NTERA-2 embryonal carcinoma cells, but not in negative control cells.
Target Information
POU5F1 (Oct-4) is a maternally expressed octamer-binding transcription factor essential for early embryonic development.
Its role in maintaining pluripotency makes it a key factor in stem cell and induced pluripotent stem (iPS) cell research.
Expression of POU5F1 along with Sox2, Klf4, Lin28, and c-Myc enables the generation of iPS cells from somatic cells without viral vectors, enhancing safety for regenerative medicine applications.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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