Thermo Fisher Scientific DNA-PKcs Polyclonal Antibody
DNA 손상 반응 경로의 핵심 단백질인 DNA-PKcs를 인식하는 Rabbit Polyclonal 항체. Western blot, IHC, IP 등에 사용 가능. 4°C 보관, 액상 형태로 제공. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:100–1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunoprecipitation (IP) | 3 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residues 2100 and 2150 of human DNA-Dependent Protein Kinase, catalytic subunit |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Target Information
DNA-PK is a heterotrimeric, DNA-dependent protein kinase that initiates signaling cascades in response to DNA damage. It is an atypical kinase closely related to the PI3 kinases and can be inhibited by small molecules targeting PI3 kinases. DNA-PK encodes a component of the mediator complex, a transcriptional coactivator complex required for expression of many genes. The complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, possibly by interacting with RNA polymerase II and promoting pre-initiation complex formation. Multiple transcript variants encoding different isoforms have been identified.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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