Thermo Fisher Scientific RPN1 Monoclonal Antibody (OTI5B1), TrueMAB
Human RPN1 단백질을 인식하는 Mouse IgG1 단일클론 항체로, WB, IHC, ICC, Flow Cytometry에 사용 가능. Lyophilized 형태이며 carrier-free로 제공되어 conjugation 실험에 적합. 고순도 Affinity chromatography로 정제됨.
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Thermo Fisher Scientific RPN1 Monoclonal Antibody (OTI5B1), TrueMAB
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI5B1 |
| Immunogen | Full length human recombinant protein of RPN1 produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
Ribophorins 1 and 2 are abundant, highly conserved glycoproteins located in the endoplasmic reticulum and participate in ribosome binding.
Mammalian oligosaccharyltransferase activity is associated with a complex composed of RPN1, RPN2, and oligosaccharyltransferase protein.
RPN1 is a component of the proteasome base; the ubiquitin-like (UBL) domain of Rad23 interacts with proteasomes via the leucine-rich repeat domain of RPN1.
The RPN1 gene maps to chromosome 3, and RPN2 maps to chromosome 20.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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