Thermo Fisher Scientific VAMP2 Recombinant Rabbit Monoclonal Antibody (ARC0936)
VAMP2 단백질을 인식하는 토끼 단클론 항체로, Western blot, ICC/IF, ELISA에 적합합니다. HEK293 세포에서 발현된 재조합 항체이며, 고순도의 Affinity Chromatography 정제 제품입니다. 인간, 생쥐, 랫트 시료에 반응하며, -20°C에서 보관합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:4,000 |
| Immunocytochemistry (ICC/IF) | 1:200–1:2,000 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | ARC0936 |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 1–100 of human VAMP2 (P63027) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.6 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol, 0.05% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2849370 |
Product Specific Information
Immunogen sequence:
MSATAATAPP AAPAGEGGPP APPPNLTSNR RLQQTQAQVD EVVDIMRVNV DKVLERDQKL SELDDRADAL QAGASQFETS AAKLKRKYWW KNLKMMIILG
Target Information
The vesicle associated membrane proteins (VAMP) or synaptobrevins are calcium-binding proteins specific to eukaryotes. VAMPs, along with synaptosomal associated protein of 25 kDa (SNAP-25) and syntaxin, form the core complex of soluble NSF attachment protein receptor (SNARE) proteins that interact with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and alpha-SNAP. These membrane-associated proteins play a key role in the regulation of vesicle membrane fusion with the plasma membrane.
The Clostridium tetani neurotoxin is a metalloprotease with specificity for VAMP. In Alzheimer’s disease, VAMP levels of all isoforms appear to be significantly lowered. It is suggested that VAMP-2 is a resident protein of the insulin-sensitive glucose transporter type 4 (GLUT4) compartment and that it is required for GLUT4 vesicle incorporation into the cell surface in response to insulin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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