Thermo Fisher Scientific CD10 Monoclonal Antibody (eBioCB-CALLA (CB-CALLA)), FITC, eBioscience
인간 CD10 단백질을 인식하는 FITC 결합 단일클론 항체로, 세포 표면 발현 분석에 적합합니다. Flow cytometry에 최적화되어 있으며, 고순도 친화 크로마토그래피 정제 제품입니다. 4°C에서 보관하며 냉동 금지. 연구용으로만 사용 가능합니다.
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- 11-0106-42
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1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD10 Monoclonal Antibody (eBioCB-CALLA (CB-CALLA)), FITC, eBioscience
Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 5 µL (0.125 µg)/test | View 5 publications |
| ELISA | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG2b, kappa |
| Recommended Isotype Control | Mouse IgG2b kappa Isotype Control (eBMG2b), FITC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBioCB-CALLA (CB-CALLA) |
| Conjugate | FITC |
| Excitation/Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10668714 |
Available Formats
- Unconjugated
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- NovaFluor Blue 610-70S
- NovaFluor Blue 660-120S
- NovaFluor Red 700
- NovaFluor Yellow 610
- NovaFluor Yellow 660
- PE
- PE-Cyanine5
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Super Bright 702
- Super Bright 780
- Request custom conjugation
Product Specific Information
The eBioCB-CALLA monoclonal antibody recognizes human CD10 (CALLA, NEP, enkephalinase, Neprilysin), a 100 kDa type II cell surface glycoprotein originally identified on most acute lymphoblastic leukemias (ALL). CD10 is a Zn²⁺-dependent metallo-peptidase involved in regulation of chemotactic and inflammatory processes involving neutrophils. It also plays a role in stromal cell-dependent B lymphopoiesis and is expressed on mature B cells in germinal centers, as well as in enterocytes and renal tubular cells.
Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-titrated and tested on normal human peripheral blood cells. Recommended usage: 5 µL (0.125 µg) per test in 100 µL total volume.
Excitation: 488 nm
Emission: 520 nm
Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD10 (Common Acute Lymphocytic Leukemia Antigen, CALLA) is a cell surface enzyme with neutral metalloendopeptidase activity that inactivates various biologically active peptides. It is expressed on lymphoblastic, Burkitt’s, and follicular germinal center lymphomas, as well as immature B cells in adult bone marrow and CML cells. CD10 is also found in breast myoepithelial cells, bile canaliculi, fibroblasts, and highly expressed in kidney and gut epithelial brush borders.
CD10 cleaves peptides at the amino side of hydrophobic residues, inactivating peptide hormones such as glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin. It is a 100 kDa type II transmembrane glycoprotein encoded by a gene with multiple alternatively spliced 5′ untranslated regions.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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